منابع مشابه
Temperature dependence of protein folding kinetics in living cells.
We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48 °C. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces in about two min. We find that this method yields impro...
متن کاملOxidative Protein-Folding Systems in Plant Cells
PLANTS ARE UNIQUE AMONG EUKARYOTES IN HAVING EVOLVED ORGANELLES the protein storage vacuole, protein body, and chloroplast. Disulfide transfer pathways that function in the endoplasmic reticulum (ER) and chloroplasts of plants play critical roles in the development of protein storage organelles and the biogenesis of chloroplasts, respectively. Disulfide bond formation requires the cooperative f...
متن کاملProtein folding and the robustness of cells
The intricate intracellular infrastructure of all known life forms is based on proteins. The folded shape of a protein determines both the protein's function and the set of molecules it will bind to. This tight coupling between a protein's function and its interconnections in the molecular interaction network has consequences for the molecular course of evolution. It is also counter to human en...
متن کاملProtein Stability, Folding, Disaggregation and Etiology of Conformational Malfunctions
Estimation of protein stability is important for many reasons: first providing an understanding of the basic thermodynamics of the process of folding, protein engineering, and protein stability plays important role in biotechnology especially in food and protein drug design. Today, proteins are used in many branches, including industrial processes, pharmaceutical industry, and medical fields. A...
متن کاملChemical labeling of protein in living cells.
Fluorescence imaging of fluorophore-labeled proteins opens a window into cellular protein biochemistry and enables direct visualization of protein dynamics, localization, and interactions in single living cells. Green fluorescent protein (GFP) and its color variants are popular fluorescent tags because they can be genetically fused to any protein of interest with great specificity. 2] However, ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.239